Axis of Awesome, a comedy trio featuring Raskopoulos, Lee Naimo, and Benny Davis, are best known for their viral "Four Chords" song and have toured internationally.
Raskopoulos told BuzzFeed News she hopes her story can help other transgender people.
"When I was growing up there was no one like me in the media that I could look up to," she said. "I want to be, for others, the person that I needed when I was young."
“Gender dysphoria is something I can remember dealing with ever since I was a kid, but I learned early on to be ashamed of it, and keep it a secret," Raskopoulos said.
While on tour in the UK in 2014, Raskopoulos was sitting in a hotel room when she realised she had to transition.
She initially thought she might have to give up performing. However, after finding inspiration in the stories of other prominent trans women in entertainment – including film director Lana Wachowski and Against Me! frontwoman Laura Jane Grace – Raskopoulos decided to keep going full steam ahead with her Axis of Awesome career.
Raskopoulos told BuzzFeed News she has "no doubt" that Axis of Awesome fans will accept her.
"They're amazing and I'm overjoyed with the support I've received since releasing the video," she said.
"But you can't release anything on the internet without risking the attention of a few trolls and it was important for me to get a word in before the haters."
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